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Leica CM 3050 S – Cryostat
5.5.2 Freezing specimens onto specimen
In instruments with specimen cooling, the 90 °
prism, which is inserted into the specimen head,
is an additional option to freeze specimens at
maximum temperature .
Installing the 90 ° prism
• Loosen screw (3) at specimen head.
• Insert pin (1) of prism completely into recep-
tacle (2) in the specimen head.
Important: the entire rear surface of the
prism must fit tightly against the specimen
head surface!
• Retighten screw (3) at specimen head.
The prism now forms a horizontal surface for
quick specimen freezing.
Freezing specimens with the 90 ° prism
• Apply sufficient amount of embedding medi-
um onto ambient temperature specimen disc,
add specimen and orient.
• Insert specimen disc with specimen into the
receptacle (4) of the prism.
• Press
to activate menu item ‘Fast Object
Freezing’ and activate function by pressing
(see chapter 5.3.4 ‘Menu functions’ item 4).
5. Operation
4
discs with 90 ° prism
(Instruments w/specimen cooling only)
The specimen head is now automatically cooled
down to the lowest temperature (-50 °C).
• Once the specimen is frozen, remove the
disc from the prism and store it in the quick-
freeze shelf until inserting it into the speci-
men head.
• Press
again to activate menu item ‘Fast
Object Freezing’ and deactivate the function
by pressing
once again (see chapter
5.3.4 ‘Menu functions’ item 4).
The specimen head temperature now automati-
cally returns to the previously selected set val-
ue.
• Loosen screw (3) at specimen head and re-
move prism (1).
• Store prism inside the cryochamber.
5.5.3 Optional accessories for freezing
Mobile heat extractor
In addition to the stationary heat extractor (part
of standard delivery), a mobile heat extractor is
available as an optional accessory - see Ap-
pendix ‘Ordering information - accessories ):
• Store heat extractor in chamber to precool.
• Place onto specimen in quick-freeze shelf.
• Remove as soon as specimen is frozen.
specimens onto specimen discs
and for removing frozen specimens