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Leica CM1950 Cryostat
• The Peltier element provides additional cooling for the freezing stations.
After the button is pressed, the display changes from "PE" to 10",
indicating an additional cooling period of 10 minutes. The remaining cool-
ing time is always shown in the display. Once only 4 minutes are remain-
ing, a dot will appear after the "4". As of this time, the Peltier element may
be switched off early by pressing the button again.
Caution:
The specimen head and Peltier do not switch on until the chamber
temperature reaches -5°C, in order to prevent icing.
• Press the "VAC" button to enable the vacuum extractor. The LED in the
"VAC" button is lit while the extractor is on. Press the button again to
disable it.
• Use the knob to adjust the intensity of the vacuum.
A Optimal area for trimming and sectioning
• Trimming: Handwheel position 12 - 6 o’clock, valve open
Handwheel position 6 - 12 o’clock, valve closed
• Cutting: Handwheel position 12 - 3 o’clock, valve open all the way
Handwheel position 3 - 6 o’clock, valve half open
Handwheel position 6 - 12 o’clock, valve closed
B Optimal area for extraction from the chamber
• To clean the chamber, turn the knob to the red range.
7. Instrument Controls
Position
12 o’clock
6 o’clock
A
B
The strength of the required extraction force depends on the following:
• Size of the specimen
• Sectioning speed
• Section thickness used
(For exact instructions for using the chamber, specimen head and
real time display fields, refer to the chapter on "Working with the
Instrument" on page 37 of this Operating Manual.)
If the condenser (resting phase) is off and the Peltier is activated,
the digit 10 flashes until the condenser switches on again to pre-
vent the Peltier from being destroyed when the condenser is not
running. When the condenser starts up, the flashing starts and the
10 minutes are counted down.