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Leica CM1950 Cryostat
8. Working with the Instrument
• Activate trimming mode.
• Select the trimming thickness.
• Switch on the VAC and select a low extraction level (between 1 and 2).
• Start trimming by manually moving the handwheel or press the
RUN/STOP and ENABLE buttons simultaneously to start motorized
sectioning.
• Use a brush to apply the section to a precooled specimen slide, then
warm it from below using your finger.
• Move the knife guard forward after removing the section.
• Remove the knife or disposable blade (using the blade ejector!).
• Insert the knife into the knife case.
8.4.3 Cutting with Extraction – Anti-roll Guide installed
• Shut off VAC (LED in VAC button goes out).
• Switch from trimming to sectioning mode (important for section stretch-
ing, as the valves work differently than in trimming mode.)
• Set the desired section thickness.
• Switch on the VAC and begin at level 1. If the section does not stretch
correctly, turn the VAC knob higher in small increments.
• Once the desired section is on the pressure plate, switch off the VAC!
Carefully fold the anti-roll guide to the side and remove the section from
the side.
After removing the section, wipe off moisture/condensate from the
pressure plate – otherwise, the next sections will become
jammed.
Cutting with extraction without anti-roll guide (brush technique) is
not possible, as the position of the pressure plate means that no
suitable air flow is obtained.