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Operating Manual V1.1 – 05/2007
Problem Causes Remedies
Blunt knife.
Inappropriate section thickness.
Incorrect clearance angle.
Microtome not dried thoroughly
enough.
Dried specimen.
Anti-roll plate is too warm or
incorrectly positioned.
Fat on the corner or edge of anti-roll
plate.
Anti-roll plate not correctly fixed.
Rust on the knife.
Anti-roll plate too warm.
Temperature too low for the tissue cut.
Blunt part, dirt, dust, frost or rust on
the knife.
Top edge of the anti-roll plate
damaged.
Hard particles in the tissue.
Dirt on knife back.
Power plug not properly connected.
Defective fuses, or circuit breaker has
triggered.
Moisture on the underside cause the
specimen to freeze to the freezing
shelf or specimen head.
Use different part of the knife.
Select correct section thickness.
Set correct angle.
Dry microtome.
Prepare new specimen.
Cool down anti-roll plate, or
reposition correctly.
Remove fat from anti-roll plate.
Fix correctly.
Remove rust.
Cool down anti-roll plate.
Increase temperature and wait.
Remove cause.
Replace anti-roll plate.
- - -
Clean.
Check if properly connected.
Replace fuses, or switch circuit
breaker back on. If not possible,
call technical service.
Apply concentrated alcohol to the
contact point.
Tissue sticks to the anti-roll
plate
Flattened sections curl up
when anti-roll plate is
folded up
Sections tear
Cryostat non-operational
Specimen disc cannot be
removed
9. Troubleshooting