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Leica CM1950 Cryostat
8. Working with the Instrument
After trimming or sectioning:
Specimen:
• Unclamp and thaw.
• Immerse in fixative for further processing.
Cleaning
• Use the brush to sweep up the section waste (section waste tray) and
remove it from the cryostat (follow applicable laboratory regulations for
disposal).
Or:
• Clean the cryostat chamber using the chamber suction nozzle:
• To do so, turn the (flat) suction nozzle of the extraction hose by
holding the hose on the white adapter and removing it with a quick
twist. "Park" the flat suction nozzle in a designated place in the
chamber, e.g. on the right inside wall of the cryogenic chamber.
• Remove the chamber suction nozzle from the plastic clip and
attach it firmly to the white adapter.
• Check the remaining capacity of the filter (in the interior of the chamber)
and change the filter if necessary (see page 28).
• Check the bacterial filter (in the front of the unit, see page 28), change it
at least every 3 months.
• Move the anti-roll guide to the side.
• Spray the cryostat chamber using Cryofect.
• Activate the UVC disinfection.