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Leica CM1950 Cryostat
49
Problem Causes Remedies
Ridged sections
Chatter during sectioning
Condensation on anti-roll
plate and knife during
cleaning
Anti-roll plate damaged
after adjustment
Thick-thin sections
Knife damaged.
Edge of anti-roll plate damaged.
Specimen insufficiently frozen onto
the specimen disc.
Specimen disc not clamped tightly.
Knife not clamped tightly enough.
Specimen has been sectioned too
thickly and has detached from disc.
Very hard, inhomogenous specimen.
Blunt knife.
Knife profile inappropriate for
specimen cut.
Incorrect clearance angle.
Brush, forceps and/or cloth too warm.
Anti-roll plate too high above the knife
edge. The adjustment was carried out
in the direction of the knife.
Temperature incorrect for the
tissue cut.
Knife profile inappropriate for the
specimen cut.
Ice buildup on the knife back.
Handwheel speed not uniform.
Knife not clamped tightly enough.
Specimen disc not clamped tightly
enough.
Cryocompound applied to cold
specimen disc; specimen detached
from disc after freezing.
Use different part of the knife.
Replace anti-roll plate.
Refreeze specimen onto the disc.
Check disc clamping.
Check knife clamping.
Refreeze specimen onto the disc.
Increase section thickness; reduce
specimen surface area if ecessary.
Use different part of the knife.
Use knife with different profile.
Set correct angle.
Store all tools on storage shelf in
the cryochamber.
Replace anti-roll plate.
Be more careful next time!
Select correct temperature.
Use knife with different profile
(c or d).
Remove ice.
Adapt speed.
Check knife clamping.
Check disc clamping.
Apply cryocompound to warm disc,
mount specimen and freeze.
9. Troubleshooting