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Chapter 3 First Light 31
Cleans and Skips tab page (Setup|Hardware): Default
Experiment Setup Main tab page (Acquisition|Experiment Setup…):
• Exposure Time: 100 ms
• Accumulations & Number of Images: 1
Experiment Setup ROI tab page (Acquisition|Experiment Setup…): Use
this function to define the region of interest (ROI).
• Imaging Mode: Selected
• Clicking on Full loads the full size of the chip into the edit boxes.
Experiment Setup Timing tab page (Acquisition|Experiment Setup…):
• Timing Mode: Free Run
• Shutter Control: Normal
• Safe Mode vs. Fast Mode: Safe
Acquiring Data
1. If you are using WinView/32 and the computer monitor for focusing, select
Focus
from the Acquisition menu. Successive images will be sent to the monitor as
quickly as they are acquired.
2. Adjust the lens aperture, intensity scaling, and focus for the best image as viewed on
the computer monitor. Some imaging tips follow:
• Begin with the lens blocked off and then set the lens at the smallest possible
aperture (largest f-stop number).
• Make sure there is a suitable target in front of the lens. An object with text or
graphics works best. If working with a microscope, use any easily viewed
specimen.
• Adjust the intensity scaling and lens aperture until a suitable setting is found. An
initial intensity scaling setting of 4096 (for a 12-bit A/D) or 65536 (for a 16-bit
A/D) assures that the image won’t be missed altogether but could be dim. Once
you’ve determined that the image is present, select a lower setting for better
contrast. Check the brightest regions of the image to determine if the A/D
converter is at full-scale. A 12-bit A/D is at full scale when the brightest parts of
the image reach an intensity of 4095. A 16-bit A/D is at full scale when the
brightest parts of the image reach an intensity of 65535.Adjust the aperture to
where it is just slightly smaller (higher f-stop) than the setting where maximum
brightness on any part of the image occurs.
• Set the focus adjustment of the lens for maximum sharpness in the viewed image.
• In the case of a camera with an F-mount, the camera lens adapter itself also has a
focus adjustment. If necessary, this focus can be changed to bring the image into
range of the lens focus adjustment. Refer to the system manual for instructions on
how to do this.